Publications

2022

Novinec M, Bembič P, Janković M, Kisilak M, Kljun J, Turel I. Kinetic Characterization of Cerium and Gallium Ions as Inhibitors of Cysteine Cathepsins L, K, and S. Int J Mol Sci, 2022, 23(16), 8993, doi: 10.3390/ijms23168993.

Vah L, Medved T, Grošelj U, Klemenčič M, Podlipnik Č, Štefane B, Wagger J, Novinec M, Svete J. Regioselective synthesis of 5- and 3-hydroxy-N-aryl-1H-pyrazole-4-carboxylates and their evaluation as inhibitors of Plasmodium falciparum dihydroorotate dehydrogenase. Molecules, 2022, 27(15), 4764, doi: 10.3390/molecules27154764.

Kladnik J, Dolinar A, Kljun J, Perea D, Grau-Exposito J, Genesca M, Novinec M, Buzon MJ, Turel I. Zinc pyrithione is a potent inhibitor of PLPro and cathepsin L enzymes with ex vivo inhibition of SARS-CoV-2 entry and replication. J Enzyme Inhib Med Chem, 2022, 37(1), 2158-2168, doi: 10.1101/2022.03.03.482819.

Varda N, Novinec M. Evolutionary Analysis of Dipeptidyl Peptidase I. Int J Mol Sci, 2022, 23(3), 1852, doi: 10.3390/ijms23031852.

Živič Z, Strmšek Ž, Novinec M, Lah J, Hadži S. Structural polymorphism of coiled-coils from the stalk domain of SARS-CoV-2 spike protein. FASEB J, 2022, 36, e22199, doi: 10.1096/fj.202101670R.

2021

Goričan T, Ciber L, Petek N, Svete J, Novinec M. Synthesis and kinetic characterization of hyperbolic inhibitors of human cathepsins K and S based on a succinimide scaffold. Bioorg Chem. 2021, 115, 105213. doi: 10.1016/j.bioorg.2021.105213.

Ulčakar L, Novinec M. Inhibition of human cathepsins B and L by caffeic acid and its derivatives. Biomolecules. 2021, 11, 31. doi: 10.3390/biom11010031.

2019

Rebernik M, Snoj T, Klemenčič M, Novinec M. Interplay between tetrameric structure, enzymatic activity and allosteric regulation of human dipeptidyl-peptidase I. Arch Biochem Biophys, 2019, 675, 108121, doi: 10.1016/j.abb.2019.108121.

Strašek N, Lavrenčič L, Oštrek A, Slapšak D, Grošelj U, Klemenčič M, Brodnik Žugelj H, Wagger J, Novinec M, Svete J. Tetrahydro-1H,5H-pyrazolo[1,2-a]pyrazole-1-carboxylates as inhibitors of Plasmodium falciparum dihydroorotate dehydrogenase. Bioorg Chem. 2019, 89, 102982. doi: 10.1016/j.bioorg.2019.102982

Rebernik M, Lenarčič B, Novinec M. The catalytic domain of cathepsin C (dipeptidyl-peptidase I) alone is a fully functional endoprotease. Protein Expr Purif. 2019, 157, 21-27. doi: 10.1016/j.pep.2019.01.009

Petek N, Štefane B, Novinec M, Svete J. Synthesis and biological evaluation of 7-(aminoalkyl)pyrazolo[1,5-a]pyrimidine derivatives as cathepsin K inhibitors. Bioorg Chem. 2019, 84, 226-238. doi: 10.1016/j.bioorg.2018.11.029

2018

Porodko A, Cirnski A, Petrov D, Raab T, Paireder M, Mayer B, Maresch D, Nika L, Biniossek ML, Gallois P, Schilling O, Oostenbrink C, Novinec M, Mach L. The two cathepsin B-like proteases of Arabidopsis thaliana are closely related enzymes with discrete endopeptidase and carboxydipeptidase activities. Biol Chem. 2018, 399(10), 1223-1235. doi: 10.1515/hsz-2018-0186

Lukić B, Grošelj U, Novinec M, Svete J. Synthesis of novel 5-(N-Boc-N-benzyl-2-aminoethyl)-7-oxo- 4, 7-dihydropyrazolo[1,5-a]pyrimidin-3-carboxamides and their inhibition of cathepsins B and K. Acta Chim Slov. 2017, 64(4), 782-789. doi: 10.17344/acsi.2017.3421.

Goričan T, Novinec M. Uravnavanje encimov s ciljanjem alosteričnih mest : primer katepsina K. Proteus : ilustriran časopis za poljudno prirodoznanstvo. 2018, 80(6), 265-271, 287. [in Slovene]

Petek N, Novinec M, Svete J. Novi derivati 5-aminoalkil-7-okso-4,7-dihidropirazolo[1,5-a]pirimidin-3-karboksilnih kislin kot inhibitorjev katepsina K in postopek priprave : SI25288 (A), 2018-04-30. Ljubljana: Urad RS za intelektualno lastnino, 2018. 17 f., 1 f. pril. [patent] [in Slovene]

2017

Hira VVV, Verbovšek U, Breznik B, Srdič M, Novinec M, Kakar H, Wormer J, van der Swaan B, Lenarčič B, Juliano L, Mehta S, van Noorden CJF, Lah Turnšek T. Cathepsin K cleavage of SDF-1[alpha] inhibits its chemotactic activity towards glioblastoma stem-like cells. Biochim Biophys Acta Mol Cell Res. 2017, 1864(3), 594-603. doi: 10.1016/j.bbamcr.2016.12.021.

Paireder M, Tholen S, Porodko A, Biniossek ML, Mayer B, Novinec M, Schilling O, Mach L. The papain-like cysteine proteinases NbCysP6 and NbCysP7 are highly processive enzymes with substrate specificities complementary to Nicotiana benthamiana cathepsin B. Biochim Biophys Acta Proteins Proteom, 2017, 1865(4), 444-452, doi: 10.1016/j.bbapap.2017.02.007.

Novinec M. Computational investigation of conformational variability and allostery in cathepsin K and other related peptidases. PLoS One, 2017, 12(8), e0182387, doi: 10.1371/journal.pone.0182387.

2016

Paireder M, Mehofer U, Tholen S, Porodko A, Schähs P, Maresch D, Biniossek ML, van der Hoorn R, Lenarčič B, Novinec M, Schilling O, Mach L. The death enzyme CP14 is a unique papain-like cysteine proteinase with a pronounced S2 subsite selectivity. Arch Biochem Biophys, 2016, 603, 110-117, doi: 10.1016/j.abb.2016.05.017

Niemer M, Mehofer U, Verdianz M, Porodko A, Schähs P, Kracher D, Lenarčič B, Novinec M, Mach L. Nicotiana benthamiana cathepsin B displays distinct enzymatic features which differ from its human relative and aleurain-like protease. Biochimie, 2016, 122, 119-125. doi: 10.1016/j.biochi.2015.06.017.

Novinec M, Rebernik M, Lenarčič B. An allosteric site enables fine-tuning of cathepsin K by diverse effectors. FEBS Lett, 2016, 590(24), 4507-4518, doi: 10.1002/1873-3468.12495.

2015

Korenč M, Lenarčič B, Novinec M. Human cathepsin L, a papain-like collagenase without proline specificity. FEBS J, 2015, 282(22), 4328-4340, doi: 10.1111/febs.13499.

Klemenčič M, Novinec M, Dolinar M. Orthocaspases are proteolytically active prokaryotic caspase homologues: the case of Microcystis aeruginosaMol Microbiol, 2015, 98(1), 142-150, doi: 10.1111/mmi.13110.

2014

Novinec M, Lenarčič B, Turk B. Cysteine cathepsin activity regulation by glycosaminoglycans. Biomed Res Int, 2014, 2014, 309718, doi: 10.1155/2014/309718.

Novinec M, Lenarčič B, Baici A. Probing the activity modification space of the cysteine peptidase cathepsin K with novel allosteric modifiers. PLoS One, 2014, 9(9), e106642, doi: 10.1371/journal.pone.0106642

Novinec M. Objava v Nature communications Nov pristop k računalniško podprtemu načrtovanju učinkovin : nov pristop v spopadu z osteoporozo?. Delo, 27. feb. 2014, letn. 56, št. 48, str. 14. [in Slovene]

Novinec M, Korenč M, Caflisch A, Ranganathan R, Lenarčič B, Baici A. A novel allosteric mechanism in the cysteine peptidase cathepsin K discovered by computational methods. Nat Commun, 2014, 5, 3287, doi: 10.1038/ncomms4287

2013

Novinec M, Lenarčič B. Cathepsin K: a unique collagenolytic cysteine peptidase. Biol Chem, 2013, 394(9), 1163-1179, doi: 10.1515/hsz-2013-0134

Novinec M, Lenarčič B. Papain-like peptidases: structure, function, and evolution. Biomol Concepts, 4(3), 287-308, doi: 10.1515/bmc-2012-0054

Klemenčič M, Novinec M, Maier S, Hartmann U, Lenarčič B. The heparin-binding activity of secreted modular calcium-binding protein 1 (SMOC-1) modulates its cell adhesion properties. PLoS One, 2013, 8(2), e56839, doi: 10.1371/journal.pone.0056839.

Baici A, Novinec M, Lenarčič B. Kinetics of the interaction of peptidases with substrates and modifiers. V: BRIX, Klaudia (ur.), STÖCKER, Walter (ur.). Proteases: structure and function. Wien: Springer-Verlag. cop. 2013, p. 37-84, doi: 10.1007/978-3-7091-0885-7_2 [book chapter]

2012

Torkar A, Bregant S, Devel L, Novinec M, Lenarčič B, Lah Turnšek T, Dive V. A novel photoaffinity-based probe for selective detection of cathepsin L active form. ChemBioChem, 2012, 13(17), 2616-2621, doi: 10.1002/cbic.201200389.

Novinec M, Lenarčič B, Baici A. Clusterin is a specific stabilizer and liberator of extracellular cathepsin K. FEBS Lett, 2012, 586(7), 1062-1066, doi: 10.1016/j.febslet.2012.03.004.

Novinec M, Pavšič M, Lenarčič B. A simple and efficient protocol for the production of recombinant cathepsin V and other cysteine cathepsins in soluble form in Escherichia coliProtein Expr Purif, 82(1), 1-5, doi: 10.1016/j.pep.2011.11.002

2010

Novinec M, Kovačič L, Lenarčič B, Baici A. Conformational flexibility and allosteric regulation of cathepsin K. Biochem J, 2010, 429(2), 379-389, doi: 10.1042/BJ20100337.

Kovačič L, Novinec M, Petan T, Križaj I. Structural basis of the significant calmodulin-induced increase in the enzymatic activity of secreted phospholipases A2Protein Eng Des Sel, 2010, 23(6), 479-487, doi: 10.1093/protein/gzq019.

2009

Kovačič L, Novinec M, Petan T, Baici A, Križaj I. Calmodulin is a non-essential activator of secretory phospholipase A2. Biochemistry, 2009, 48(47), 11319-11328, doi: 10.1021/bi901244f

2008

Bubik A, Sedmak B, Novinec M, Lenarčič B, Lah Turnšek T. Cytotoxic and peptidase inhibitory activities of selected non-hepatotoxic cyclic peptides from cyanobacteria. Biol Chem, 389(10), 1339-1346, doi: 10.1515/BC.2008.153.

Novinec M, Kovačič L, Škrlj N, Turk V, Lenarčič B. Recombinant human SMOCs produced by in vitro refolding: calcium-binding properties and interactions with serum proteins. Protein Expr Purif, 62(1), 75-82, doi: doi:10.1016/j.pep.2008.07.009.

2007

Novinec M, Grass RN, Stark WJ, Turk V, Baici A, Lenarčič B. Interaction between human cathepsins K, L, and S, Mechanism of elastinolysis and ihibition by macromolecular inhibitors. J Biol Chem, 2007, vol. 282, no. 11, p. 7893-78902.  doi: 10.1074/jbc.M610107200

2006

Novinec M, Kordiš D, Turk V, Lenarčič B. Diversity and evolution of the thyroglobulin type-1 domain superfamily. Mol Biol Evol, 2006, vol. 23, p. 744-755.  doi: 10.1093/molbev/msj082

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